Difference between revisions of "840:153g:Projects/project23/2012/11/02"

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(Autocreate 2012/11/02 Entry for 840:153g:Projects/project23)
 
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==Entry title==
 
==Entry title==
* Insert content here...
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* Today, we photographed the gel from 11.01.2012. After photographing both gels, we cut out the bands from the 350bp and 450bp regions.  We stored these gel slices at -20°C for further DNA extraction next week.
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*1 percent agarose gel
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*1. 100-base pair ladder
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*2-4. Replicates of primered set
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[[image:11.09.12_gel_1.0.jpg]]
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*Conclusion: These samples banded like all others before.  We can use this for further extraction.
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*1.8 percent agarose gel
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##Positive control
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##100 base-pair ladder
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##sfmr frag w/o DNA (neg control)
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##primered set w/o DNA (neg control)
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##mfsr frag w/o DNA (neg control)
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##100 base-pair ladder
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##sfmr frag w/ DNA
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##primered set w/ DNA
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##mfsr frag w/ DNA
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##100 base-pair ladder
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##primered replicate
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##primered replicate
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[[image:11.09.12_gel_1.8.jpg|600px]]
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*Conclusion: Positive and negative controls check ok. For comparison, the primered set does have new, distinct banding from both fragments.  After further calculations, our total product length should be ~350bp.  A previous calculation did not take the overlap region between fragments into consideration.  We will extract both the 350 and 450-bp regions to be certain.  The replicates also look usable in this gel.
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[[category:OWWLabNotebookV1]]
 
[[category:OWWLabNotebookV1]]

Revision as of 18:48, 8 November 2012

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Entry title

  • Today, we photographed the gel from 11.01.2012. After photographing both gels, we cut out the bands from the 350bp and 450bp regions. We stored these gel slices at -20°C for further DNA extraction next week.


  • 1 percent agarose gel
  • 1. 100-base pair ladder
  • 2-4. Replicates of primered set

11.09.12 gel 1.0.jpg

  • Conclusion: These samples banded like all others before. We can use this for further extraction.


  • 1.8 percent agarose gel
    1. Positive control
    2. 100 base-pair ladder
    3. sfmr frag w/o DNA (neg control)
    4. primered set w/o DNA (neg control)
    5. mfsr frag w/o DNA (neg control)
    6. 100 base-pair ladder
    7. sfmr frag w/ DNA
    8. primered set w/ DNA
    9. mfsr frag w/ DNA
    10. 100 base-pair ladder
    11. primered replicate
    12. primered replicate

11.09.12 gel 1.8.jpg

  • Conclusion: Positive and negative controls check ok. For comparison, the primered set does have new, distinct banding from both fragments. After further calculations, our total product length should be ~350bp. A previous calculation did not take the overlap region between fragments into consideration. We will extract both the 350 and 450-bp regions to be certain. The replicates also look usable in this gel.