Difference between revisions of "840:153g:Projects/project23/2012/10/26"

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(Site-directed Mutagenesis)
(Site-directed Mutagenesis)
Line 30: Line 30:
  
 
*Results:  
 
*Results:  
*Upper lane, wells 1-5
+
*Upper lane, wells 1-5 (no DNA set)
 +
[[Image:10.26.12_Control_Lanes.jpg|600px]]
 +
 
 +
 
 +
*Upper lane, wells 5-10
 +
[[Image:10.26.12_Sample_Lanes.jpg|600px]]
 +
 
 +
 
 +
*Lower lane
 +
[[Image:10.26.12_Replicates.jpg|600px]]
  
  

Revision as of 16:34, 29 October 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Site-directed Mutagenesis

  • On Friday, we ran Thursday's PCR products on a 1.8% Agarose gel. The samples were loaded as follows:


  • Upper lane
  1. 100bp ladder
  2. Non-primered set without template
  3. Upstream fragment without template
  4. Primered set without template
  5. Downstream fragment without template
  6. 100 bp ladder
  7. Downstream fragment
  8. Primered set
  9. Upstream fragment
  10. Non-primered set


  • Lower lane
  1. Positive control
  2. 100 bp ladder
  • All other lanes are replications of primered set


  • Results:
  • Upper lane, wells 1-5 (no DNA set)

10.26.12 Control Lanes.jpg


  • Upper lane, wells 5-10

10.26.12 Sample Lanes.jpg


  • Lower lane

10.26.12 Replicates.jpg