840:153g:Projects/project23/2012/10/25: Difference between revisions
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==Continuation of the Mutagenesis Reaction == | ==Continuation of the Mutagenesis Reaction == | ||
* On Tuesday, we ran the gel for last week's PCR products. | * On Tuesday, we ran the gel for last week's PCR products. The high concentration of 1:20 template DNA, and a low concentration of 1:200 were prepared with water for each the upstream and downstream fragments. Our nomenclature used is three-part, and outlined below. | ||
*1.)Primer configuration | |||
**N = no primers used | |||
**P = outside primers used | |||
*2.)Template DNA concentration | |||
** + = 1:20 | |||
** - = 1:200 | |||
*3.)Annealing temperature for PCR, in °C | |||
*Our positive control was standard template DNA and associated primers from lab stock | |||
*Our negative control had outside primers and no template | |||
*We loaded our samples as follows: | |||
*Top Lane: | *Top Lane: | ||
Line 28: | Line 42: | ||
#N+60 | #N+60 | ||
#N+65 | #N+65 | ||
# | #Negative Control | ||
#N-55 | #N-55 | ||
#N-60 | #N-60 |
Revision as of 15:14, 29 October 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Continuation of the Mutagenesis Reaction
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