Difference between revisions of "840:153g:Projects/project23/2012/10/25"

From OpenWetWare
Jump to: navigation, search
(Continuation of the Mutagenesis Reaction)
Line 7: Line 7:
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==Continuation of the Mutagenesis Reaction ==
 
==Continuation of the Mutagenesis Reaction ==
*  
+
 
 +
* On Tuesday, we ran the gel for last week's PCR products.  This was done on a dual-lane chamber, loaded as follows:
 +
 
 +
 
 +
*Top Lane:
 +
#100 bp ladder
 +
#P+55
 +
#P+60
 +
#P+65
 +
#Positive Control
 +
#P-55
 +
#P-60
 +
#P-65
 +
#100 bp ladder
 +
 
 +
 
 +
*Bottom Lane:
 +
#100 bp ladder
 +
#N+55
 +
#N+60
 +
#N+65
 +
#Positive Control
 +
#N-55
 +
#N-60
 +
#N-65
 +
#100 bp ladder
 +
 
 +
 
 +
*We ran these on a 1% agarose gel for 30min at 120V
 +
 
 +
 
 +
*Results:
 +
 
 +
 
  
  

Revision as of 13:54, 29 October 2012

Owwnotebook icon.png Cloning of the OmcF gene <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Continuation of the Mutagenesis Reaction

  • On Tuesday, we ran the gel for last week's PCR products. This was done on a dual-lane chamber, loaded as follows:


  • Top Lane:
  1. 100 bp ladder
  2. P+55
  3. P+60
  4. P+65
  5. Positive Control
  6. P-55
  7. P-60
  8. P-65
  9. 100 bp ladder


  • Bottom Lane:
  1. 100 bp ladder
  2. N+55
  3. N+60
  4. N+65
  5. Positive Control
  6. N-55
  7. N-60
  8. N-65
  9. 100 bp ladder


  • We ran these on a 1% agarose gel for 30min at 120V


  • Results: