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3Amp: 2 colonies (only one was present at the time of picking) 2Amp: 0 colonies 1Amp: 0 colonies +Amp: 20 colonies -Amp: 0 colonies -NoAmp: plate completely covered.
The top band on the ladder (first lane) represents 3000 b.p. As you can see, we have a bright band above this line (lane 2) which corresponds to the size of our T-vector (~3200 b.p.). We do not, however, see a bright line at ~1500 b.p. which we would expect with successful transformation of our gene (pigI is 1473 b.p.). To confirm these results, we will send off our DNA product for sequencing.