Difference between revisions of "840:153g:Projects/project22/2012/11/01"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2012/11/01 Entry for 840:153g:Projects/project22)
 
Line 1: Line 1:
 
{|{{table}} width="800"
 
{|{{table}} width="800"
 
|-
 
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Prodigiosin Production in E. Coli</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
+
==Genomic DNA Extraction Attempt 2==
* Insert content here...
+
* On Tuesday we began another genomic DNA Extraction of ''S. marcescens'' (a culture was grown up from a streak plate on Monday) using protocols from openwetware.org ([[http://openwetware.org/wiki/Genomic_DNA_Extraction]]). Today we ran simple gel electrophoresis on our 6 DNA samples. Unfortunately, we had no visible bands displayed.
 +
 
 +
[[Image:01.11.12_small.png‎]]
 +
 
 +
We suspect this may be because our DNA was not fully resuspended in tris buffer (there was still visible product in the tubes). On Tuesday, we will check our samples to see if there is still visible product in them and if so, we will centrifuge the tubes, pour off the supernatant into another tube and try to suspend the visible product in more tris buffer (this is in case we have too much DNA product in too little buffer). We will then run gel electrophoresis on all the samples.
  
  

Revision as of 13:51, 1 November 2012

Owwnotebook icon.png Prodigiosin Production in E. Coli <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Genomic DNA Extraction Attempt 2

  • On Tuesday we began another genomic DNA Extraction of S. marcescens (a culture was grown up from a streak plate on Monday) using protocols from openwetware.org ([[1]]). Today we ran simple gel electrophoresis on our 6 DNA samples. Unfortunately, we had no visible bands displayed.

01.11.12 small.png

We suspect this may be because our DNA was not fully resuspended in tris buffer (there was still visible product in the tubes). On Tuesday, we will check our samples to see if there is still visible product in them and if so, we will centrifuge the tubes, pour off the supernatant into another tube and try to suspend the visible product in more tris buffer (this is in case we have too much DNA product in too little buffer). We will then run gel electrophoresis on all the samples.