840:153g:Projects/project22/2012/10/25: Difference between revisions
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[[Image:25.10.12_small.jpg]] Lane 3 marker on the right to Lane 12 marker on the left | [[Image:25.10.12_small.jpg]] Lane 3 marker on the right to Lane 12 marker on the left | ||
As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results with this DNA since day 1, so we will start preparing for another DNA extraction next week. | As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week. | ||
* We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol. | * We also began collecting buffers and the necessary materials for another DNA extraction for next week, where we will use the OpenWetWare extraction protocol. |
Revision as of 14:33, 25 October 2012
Prodigiosin Production in E.Coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Attempted PCR and Gel Electrophoresis Verification
Lane 3 marker on the right to Lane 12 marker on the left As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week.
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