840:153g:Projects/project22/2012/10/04: Difference between revisions
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On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in. | On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in. | ||
Wells 1-12 were ours | |||
[[Image:http://openwetware.org/images/thumb/4/44/%21GROUP22.25O.tif/640px-%21GROUP22.25O.tif.png]] | |||
Wells 19-20 were ours (negative controls) | |||
[[Image:http://openwetware.org/images/thumb/e/ef/%21GROUP24.2.tif/640px-%21GROUP24.2.tif.png]] | |||
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Revision as of 13:19, 4 October 2012
 Prodigiosin Production by E. Coli.
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PCR Results and RetryOn Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in. Wells 1-12 were ours File:Http://openwetware.org/images/thumb/4/44/!GROUP22.25O.tif/640px-!GROUP22.25O.tif.png Wells 19-20 were ours (negative controls) File:Http://openwetware.org/images/thumb/e/ef/!GROUP24.2.tif/640px-!GROUP24.2.tif.png | |
