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Buffer preparation and starting of DNA extraction

  • On Tuesday,(September 11), two 250 Erlenmeyer flasks containing 50mL of LB and two test tubes containing 5mL of LB were inoculated with S. marcescens. These were then placed in a shaker at 37 degrees Celsius and 222rpm. We observed growth of the bacteria in the medium on Wednesday, so Thursday we began preparation for our DNA extraction. Thursday began by preparing the necessary buffers. We then spun down the 50mL flasks of bacteria, resuspended the pellet in Tris buffer, and froze them at -20 degrees Celsius.