840:153g:Projects/project20/2011/11/10

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Ligation Trial 2

  • Transformation experiment done on 11/08/11 failed.
  • We decide to increase the concentration of DNA in Ligation reaction.
  • We increse concentration to 25 times. It means 1 part of vector and 25 parts of insert.
  • After completing experiment, we realized that for first ligation experiment we have frozen ligation mix.So thats why ligation trial 1 failed.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=840:153g:Projects/project20/2011/11/10&oldid=565940"