840:153g:Projects/project20/2011/11/10

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Ligation Trial 2

  • Transformation experiment done on 11/08/11 failed.
  • We decide to increase the concentration of DNA in Ligation reaction.
  • We increse concentration to 25 times. It means 1 part of vector and 25 parts of insert.
  • After completing experiment, we realized that for first ligation experiment we have frozen ligation mix.So thats why ligation trial 1 failed.