Difference between revisions of "840:153g:Projects/project20/2011/11/10"

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(Autocreate 2011/11/10 Entry for 840:153g:Projects/project20)
 
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==Entry title==
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==Ligation Trial 2==
* Insert content here...
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* Transformation experiment done on 11/08/11 failed.
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* We decide to increase the concentration of DNA in Ligation reaction.
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* We increse concentration to 25 times. It means 1 part of vector and 25 parts of insert.
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* After completing experiment, we realized that for first ligation experiment we have frozen ligation mix.So thats why ligation trial 1 failed.
  
  

Revision as of 21:38, 10 November 2011

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Ligation Trial 2

  • Transformation experiment done on 11/08/11 failed.
  • We decide to increase the concentration of DNA in Ligation reaction.
  • We increse concentration to 25 times. It means 1 part of vector and 25 parts of insert.
  • After completing experiment, we realized that for first ligation experiment we have frozen ligation mix.So thats why ligation trial 1 failed.