Difference between revisions of "840:153g:Projects/project20/2011/10/20"

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(Autocreate 2011/10/20 Entry for 840:153g:Projects/project20)
 
(Agarose gel electrophoresis (PCR: 2nd Attempt))
 
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==Entry title==
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==Plasmid Extraction from Transformed competent cells==
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* Plasmid extraction from overnight incubated transformed ''e.coli'' plates
 
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== Agarose gel electrophoresis (PCR: 2nd Attempt)==
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* Results very familiar to 1st attempt of PCR.
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==PCR(3rd Attempt==
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* On the results on 2nd PCR attempt.We decide to next gradient pcr for our nprT Gene at higher temperature(above 48 degree Celsius)
  
 
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Latest revision as of 11:29, 8 December 2011

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Plasmid Extraction from Transformed competent cells

  • Plasmid extraction from overnight incubated transformed e.coli plates

Agarose gel electrophoresis (PCR: 2nd Attempt)

  • Results very familiar to 1st attempt of PCR.

PCR(3rd Attempt

  • On the results on 2nd PCR attempt.We decide to next gradient pcr for our nprT Gene at higher temperature(above 48 degree Celsius)