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Transforming ligations into competent cells
We transformed our plasmids into competent bacteria and plated them. We had 27 plates, 26 were LB ampicillin plates, 1 was non-ampicillin plate. Of the 26 LB ampicillin plates, 24 of them contained our our combinations of wintergreen and RFP genes. The other 2 LB ampicillin plates each contained controls, 1 had our positive control (competent plasmid), the other had our negative control (just RFP). The non-ampicillin plate was a non-selective control which is just competent cells.