Difference between revisions of "840:153g:Projects/project16/2011/10/25"

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(Gel Electrophoresis of Previous PCR Products)
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==Entry title==
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==Gel Electrophoresis of Previous PCR Products==
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Today we ran a 1% agarose gel electrophoresis of our PCR products from lab session. Because the PCR we ran was the same as the one from 10/13/2011, we expected to see the same results from our gel. As you probably can guess based on past results, it didn't work because Jack added too much ethidium bromide to the gel, causing it to turn orange as if we were going for Halloween colors.  However this made it very difficult to see the DNA ladder and the results from our lanes which looked strikingly different from what we expected from 10/13.  Based on this failure as well as other failures, we have decided to abandon this project and move on to greener pastures.
  
  

Revision as of 20:19, 25 October 2011

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Gel Electrophoresis of Previous PCR Products

Today we ran a 1% agarose gel electrophoresis of our PCR products from lab session. Because the PCR we ran was the same as the one from 10/13/2011, we expected to see the same results from our gel. As you probably can guess based on past results, it didn't work because Jack added too much ethidium bromide to the gel, causing it to turn orange as if we were going for Halloween colors. However this made it very difficult to see the DNA ladder and the results from our lanes which looked strikingly different from what we expected from 10/13. Based on this failure as well as other failures, we have decided to abandon this project and move on to greener pastures.