From OpenWetWare
Revision as of 07:41, 10 October 2011 by Kelsey Hampton (talk | contribs) (DNA Extraction and Isolation using Proteinase K)
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

DNA Extraction and Isolation using Proteinase K

Today we performed a different DNA extraction under a protocol recommended by a faculty member to hopefully obtain a better sample of DNA. We utilized a lysis buffer and proteinase K on buccal cells followed by an ethanol precipitation, but our efforts were in vain. No DNA precipitate was formed, and therefore the extraction was unsuccessful. Following this, we found a DNA extraction kit that we plan to utilize in our next lab session to perform still another DNA extraction.