Difference between revisions of "840:153g:Projects/project16/2011/10/04"

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==Entry title==
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==Gel Electrophoresis of PCR and Creating Lysis Buffer==
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Today, we ran our PCR products through a gel to determine if we successfully amplified our gene. Unfortunately, we failed to amplify our gene and we forgot to add the master mix to 2 of our 3 controls. There are many different things that could have made our PCR failed which include a failed DNA extraction, incorrect annealing temperatures, an incorrect master mix, etc.  So we elected to try a different extraction protocol that we acquired from Dr. Spradling.  In preparation for Thursday's lab, we created our lysis buffer.
  
  

Revision as of 14:37, 4 October 2011

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Gel Electrophoresis of PCR and Creating Lysis Buffer

Today, we ran our PCR products through a gel to determine if we successfully amplified our gene. Unfortunately, we failed to amplify our gene and we forgot to add the master mix to 2 of our 3 controls. There are many different things that could have made our PCR failed which include a failed DNA extraction, incorrect annealing temperatures, an incorrect master mix, etc. So we elected to try a different extraction protocol that we acquired from Dr. Spradling. In preparation for Thursday's lab, we created our lysis buffer.