Difference between revisions of "840:153g:Projects/project11/2010/11/18"

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(Autocreate 2010/11/18 Entry for 840:153g:Projects/project11)
 
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Tuesday: We digested our 9 remaining colonies yet to be screened for our (gene&double terminator)superpart and ran on a gel. results from this gel of the 9 remaining colonies resembled that from previous gels we ran. Base pair length ended up between 100-200bp so we believe there is a problem with the Ecor1 during the ligation process preventing our two parts break apart correctly in order to ligate them together.
  
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We also religated our left over Gene & double terminator parts that we had stored. In preperation for another bacteria transformation.
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Thursday: transformed bacteria with our newly religated gene & double terminator superpart. In preperation for a digestion and gel electrophoresis.
  
 
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Revision as of 13:33, 18 November 2010

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Tuesday: We digested our 9 remaining colonies yet to be screened for our (gene&double terminator)superpart and ran on a gel. results from this gel of the 9 remaining colonies resembled that from previous gels we ran. Base pair length ended up between 100-200bp so we believe there is a problem with the Ecor1 during the ligation process preventing our two parts break apart correctly in order to ligate them together.

We also religated our left over Gene & double terminator parts that we had stored. In preperation for another bacteria transformation.

Thursday: transformed bacteria with our newly religated gene & double terminator superpart. In preperation for a digestion and gel electrophoresis.