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  • Tuesday we transformed bacteria with plasmids from three different parts; promoter, RBS, and double terminator. Wednesday we counted colonies on plates and innoculated media tubes for continued growth of colonies. Innoculated 3 tubes with the promoter transformed bacertia colonies, 4 tubes with the double terminator transformed bacteria colonies, and 6 tubes with the RBS transformed bacteria colonies. 6 tubes of RBS were done because of inconsistent growth on the plate.

Thursday we perfomed a plasmid miniprep on all parts. Prepared glycerol stocks. Plasmids that were isolated were stored in -80C as well as the glycerol stocks. Next week we plan to digest the plasmids for our parts and run on gel to make sure the correct parts are in the plasmids.