840:153g:Projects/project10/2010/11/04

From OpenWetWare
Revision as of 13:52, 4 November 2010 by Lorena Christensen (talk | contribs) (Entry title)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. First two lanes are G & C primers, middle is ladder, and last two lanes are S &C primers.

File:RT Two.jpg


Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded. It did, bands were faint but showed up around 900 bp mark. Top well is S&C primers, middle is ladder, bottom is G&C primers.

File:Purification gel.jpg