From OpenWetWare
Revision as of 14:49, 4 November 2010 by Lorena Christensen (talk | contribs) (Entry title)
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. First two lanes are G & C primers, middle is ladder, and last two lanes are S &C primers. File:RT Two.jpg Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded.