Difference between revisions of "840:153g:Projects/project10/2010/11/04"

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==Entry title==
 
==Entry title==
Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded.   
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Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. First two lanes are G & C primers, middle is ladder, and last two lanes are S &C primers.
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[[Image:RT Two.jpg]]
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Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded. It did, bands were faint but showed up around 900 bp mark. Top well is S&C primers, middle is ladder, bottom is G&C primers.
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[[Image:Purification gel.jpg]]  
  
  

Revision as of 13:52, 4 November 2010

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Entry title

Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. First two lanes are G & C primers, middle is ladder, and last two lanes are S &C primers.

File:RT Two.jpg


Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded. It did, bands were faint but showed up around 900 bp mark. Top well is S&C primers, middle is ladder, bottom is G&C primers.

File:Purification gel.jpg