Difference between revisions of "840:153g:Projects/project10/2010/11/04"

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==Entry title==
 
==Entry title==
Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded.   
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Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR.[[Image:RT Two.jpg]]
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Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded.   
  
  

Revision as of 13:47, 4 November 2010

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Entry title

Discovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR.File:RT Two.jpg Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded.