Difference between revisions of "840:153g:Projects/project10/2010/09/30"

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==Entry title==
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[[Image:[[Image:9_30_10Group10.jpg]]]]==Entry title==
* This week we finished DNA extraction from P. chrysosporium and ran a gel electrophoresis to determine presence of DNA. We also figured out our primers - forward with biobrick extension, reverse with biobrick extension, forward without biobrick extension, and reverse without biobrick extension - to be ordered.  
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* This week we finished DNA extraction from P. chrysosporium and ran a gel electrophoresis to determine presence of DNA... it worked! We know it worked because we see DNA on the gel. We also figured out our primers - forward with biobrick extension "Sneezy" GTTTCTTCGAATTCGCGGCCGCTTCTAGCCTTCGTATGTAAGTCGCTG, reverse with biobrick extension "Cowgirl" TACTAGTAGCGGCCGCTGCAGGAAGAAACCGCCGTGCGCGAGTCGCGCG, forward without biobrick extension "Grumpy" CCTTCGTATGTAAGTCGCTG, and reverse without biobrick extension "Cowboy" CGCCGTGCGCGAGTCGCGCG - to be ordered.  
 
Next time we will hopefully receive our primers and run PCR to determine if the primers work.
 
Next time we will hopefully receive our primers and run PCR to determine if the primers work.
  

Revision as of 12:46, 7 October 2010

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[[Image:9 30 10Group10.jpg]]==Entry title==

  • This week we finished DNA extraction from P. chrysosporium and ran a gel electrophoresis to determine presence of DNA... it worked! We know it worked because we see DNA on the gel. We also figured out our primers - forward with biobrick extension "Sneezy" GTTTCTTCGAATTCGCGGCCGCTTCTAGCCTTCGTATGTAAGTCGCTG, reverse with biobrick extension "Cowgirl" TACTAGTAGCGGCCGCTGCAGGAAGAAACCGCCGTGCGCGAGTCGCGCG, forward without biobrick extension "Grumpy" CCTTCGTATGTAAGTCGCTG, and reverse without biobrick extension "Cowboy" CGCCGTGCGCGAGTCGCGCG - to be ordered.

Next time we will hopefully receive our primers and run PCR to determine if the primers work.