7-AAD: Difference between revisions
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(New page: 7-aminoactinomycin-D * 546/647 * Nucleic acid dye * Stains '''dead cells''' * "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluores...) |
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* Nucleic acid dye | * Nucleic acid dye | ||
* Stains '''dead cells''' | * Stains '''dead cells''' | ||
* "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter). | * "The advantage of 7-AAD over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter). | ||
Revision as of 13:52, 6 July 2008
7-aminoactinomycin-D
- 546/647
- Nucleic acid dye
- Stains dead cells
- "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
