Difference between revisions of "7-AAD"

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* 546/647
 
* 546/647
 
* Nucleic acid dye
 
* Nucleic acid dye
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* Often used in conjunction with [[Annexin-V]] when assaying for apoptotic cells
 
* "The '''advantage of 7-AAD''' over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter).
 
* "The '''advantage of 7-AAD''' over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter).
  
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Add any other categories that may be relevant to your page.  We use categories like tags.
 
Add any other categories that may be relevant to your page.  We use categories like tags.
 
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[[Category:Material]]
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[[Category:Material]] [[Category:Dye]] [[Category:Viability dye]]

Latest revision as of 05:30, 5 May 2009

7-aminoactinomycin-D

Purpose

  • Staining dead cells

Procurement

Use

  • 546/647
  • Nucleic acid dye
  • Often used in conjunction with Annexin-V when assaying for apoptotic cells
  • "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Safety

References

  • MC O’Brien, et al. (1995) Cytometry 19:243
  • I Schmid et al. (1992) Cytometry 13:204