Difference between revisions of "3-Aminotriazole"

From OpenWetWare
Jump to: navigation, search
(adapted to template)
m
 
Line 3: Line 3:
  
 
==Procurement==
 
==Procurement==
You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].
+
*You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].
  
 
==Use==
 
==Use==
Line 12: Line 12:
  
 
==References==
 
==References==
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
+
*[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
  
 
[[Category:Material]] [[Category:Chemical]]
 
[[Category:Material]] [[Category:Chemical]]

Latest revision as of 00:54, 7 March 2008

Purpose

  • 3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.

Procurement

  • You can buy 3-amino-1,2,4-triazole for example at Sigma.

Use

  • Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.

Safety

  • Possible carcinogen. Use gloves, work in a hood.

References