3-Aminotriazole: Difference between revisions
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3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies. | ==Purpose== | ||
*3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies. | |||
==Procurement== | |||
You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma]. | |||
==Use== | |||
*Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM. | |||
References | ==Safety== | ||
*Possible carcinogen. Use gloves, work in a hood. | |||
==References== | |||
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information] | [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information] | ||
[[Category:Material]] [[Category:Chemical]] | [[Category:Material]] [[Category:Chemical]] | ||
Revision as of 01:53, 7 March 2008
Purpose
- 3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.
Procurement
You can buy 3-amino-1,2,4-triazole for example at Sigma.
Use
- Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.
Safety
- Possible carcinogen. Use gloves, work in a hood.
