2020(S08) Lecture:week 5

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Week 5 Tuesday

Today will be a project work day with your advanced student mentors. You might consider reviewing the project organizer or the requirements for the 3 ideas presentation or the student resources list. There is no lecture log required for today but you should update your project development notebook, electronically if you're keeping it that way, or on paper if that's your preferred style. Your 3 ideas presentation will be one week from tomorrow.

Homework for Thursday

Read the chapter you were given written by Charles Weiner that was photocopied from the "Encyclopedia of Ethical, Legal, and Policy Issues in Biotechnology." The chapter serves three important purposes for our class. First, it provides some context for the upcoming video presentation of the 1974 Cambridge City Council Hearings. These hearings enabled the citizens of Cambridge to directly address the scientists themselves and question the intent and efficacy of national safety guidelines for recombinant DNA work. The video was made and made available to us by Charles Weiner and it will be shown on Thursday in class. Second, this chapter will give some timeline for the development of the technology itself as well as for the meetings and hearings that addressed its hazards. Finally, this chapter gives some insight into the polarizing viewpoints and biases inherent in many of the discussions associated with these issues.
There are three short parts to this assignment and you should spend no more than 1 hour reading the chapter and answering these questions.

  • Part 1: What is recombinant DNA?
To illustrate your understanding of genetic engineering techniques, describe how you might make the DNA that programs the GloFish shown here
image of GloFish from Wikipedia entry about Recombinant DNA
. Call the gene for glowing "GFG" and the fish plasmid "pFP."
  • Part 2: Key events for regulation of experiments involving recombinant DNA

Concisely describe the relevance/importance of

    • 1973 Gordon Conference
    • 1974 Berg letters in Nature and Science
    • 1975 Conference in Asilomar
    • Recombinant DNA Advisory Committee

All of these are described in Charles Weiner's chapter that you were given.

  • Part 3: Public scrutiny of and say in research decisions

Consider these three quotes and then

    • give your idea(s) about how to best engage the public in the scientific enterprise
    • comment on one author's viewpoint or agenda, as best you can glean from the short quote
    • and finally say if you think the publication was an appropriate places to express that author's viewpoint

Quote 1: from pg 910 of Encyclopedia chapter (above) "The motive from the start was to avoid public interference and to demonstrate that the scientists on their own could protect laboratory workers, the public and the environment."
Quote 2: from Open Letter to the Asilomar Conference written by Science for the People "There is little evidence that the technologies being discussed at this meeting arise from social or medical needs of large segments of the population. Rather, they represent specialized interests including those of the scientific community itself."
Quote 3: from Summary Statement of the Asilomar Conference written by Paul Berg et al and published in Proceedings of the National Academy of Science "In the longer term, serious problems may arise in the large scale application of this methodology in industry, medicine and agriculture. But it was also recognized that further research and experience may show that many of the potential biohazards are less serious and/or less probable than we now suspect."

Upload your answers to the following questions into your personal design portfolios that are here

Week 5 Studio

Today will be a second project work day with your advanced student mentors. You should organize your progress and task list and thinking in your project development notebook, be it electronic or on paper, depending on your preferred style. Your 3 ideas presentation will be one week from today.
For one hour, the advanced students will discuss a journal article. You are welcome to listen to that presentation/discussion or continue to work independently.

Week 5 Thursday

Challenge: "Refrain from using the alphabet"

Turning back the clock: it’s three years after the scientific community raised concern about the safety of recombinant DNA experiments. It’s one month after the NIH issued guidelines to regulate recombinant DNA work. The Cambridge City Council is meeting with scientists from nearby institutions to discuss the consequence of these guidelines for the community surrounding the laboratories and to consider additional resolutions and actions that the city might take to ensure the safety of its citizens. Charlie Weiner made this videotape for the the MIT Oral History Program Recombinant DNA History Project, and he has generously shared it with us. The title of the video is, *“Hypothetical Risks, The Cambridge City Council hearings on DNA experimentation in Cambridge.”
It was recorded at the City Hall Cambridge MA 1976 and though the audio and the video quality have degraded, it provides an invaluable window into the dynamics of these debates, showing the human side of what can sometimes, in retrospect look like academic debates or over-emotional public reaction.
The cast, listed in the order of speaking:

  • Mr. Alfred Vellucci, Mayor of Cambridge,
  • Dr. Mark Ptashne, Prof. of Biochemistry and Molecular Biology, Harvard University
  • Dr. Daniel Branton, Chairman Safety Committee, Harvard University
  • Dr. Maxine Singer, Biochemist, National Institutes of Heath (NIH)
  • Ms. Saundra Graham, Cambridge City Council
  • Mr. David Clem, Cambridge City Council
  • Dr. Ruth Hubbard, Prof. of Biology, Harvard University
  • Dr. Jonathan King, Prof. of Biology, MIT

Their exchanges highlight many relevant aspects of emerging technologies, not only recombinant DNA. After we watch the videotape we will consider, as a class, some of the dialog and the lessons we might apply from this history to biotechnologies coming on line today (and tomorrow). These considerations include

  1. Risk assessment
  2. Self-regulation vs government legislation
  3. Scientific process
  4. Rhetoric
  5. Past performance as indicator of future returns?
  6. Preparedness
  7. Scenario building
  8. Detection and Surveillance

Beyond keeping these topics in mind as you watch the video, you should also be familiar with the kinds of biosafety (then called biohazard) levels that will be discussed and what each level (then called P1, P2, P3, and P4; now called BSL1, 2, 3, 4) means. This can be done by watching the BSL video that is here.

After watching the videotape

As a class we will consider the following exchanges and what they tell us about the 8 considerations listed above. The quotes (as accurate as I can render them!) are presented in the order in which they appear in the videotape itself.

Videoclip 1

Mayor of Cambridge, Alfred Vellucci: “Refrain from using the alphabet. Most of us in this room including myself are laypeople. We don’t understand your alphabet. So you will spell it out for us so that we know exactly what you’re talking about because we are here to listen”

Videoclip 2

Dr. Mark Ptashne: “Let me begin by giving you a blanket statement of fact. No known dangerous organism has ever been produced by recombinant DNA experiments. …You must realize that unlike other real risks involved in experimentation, the risks in this case are purely hypothetical…I believe it to be the opinion of the overwhelming majority of microbiologists that there is in fact no significant risk involving experiments authorized to be done by the federal guidelines in P1, P2 or P3 laboratory.”

Videoclip 3

Dr. Daniel Branton: “Our awareness of these tremendous potentials clearly does not relieve us of the responsibility to exercise the utmost caution in regulating work with recombinant DNA...What we have been asked to do and what we are doing…is to regulate research that does not pose a known threat to public health and that is extremely unlikely to impose such a threat.”

Videoclip 4

Dr. Maxine Singer: “I must say that I’ve been surprised recently to find myself put among those who are considered to be unconcerned. I have been concerned and I continue to be concerned. I feel that these guidelines are a very responsible response to that concern....The whole deliberation within the NIH has been a very open process”

Homework for Tuesday

Read the photocopied handout from Jon Beckwith's book, "Making Genes Making Waves."
This chapter was chosen for you to read because it includes several important lessons drawn from the author's personal history and his career development. The chapter also includes some important scientific content about bacteriophage and E. coli's lac operon. Near the conclusion, it presents a rare, first-hand account of an emerging technology, namely direct gene manipulation, that was immediately recognized for its pioneering and frightening possibilities. As described in this chapter, Jon Beckwith's scientific work enabled the lacZ gene from E. coli to be purified, making the ability to purify any gene from E. coli seem close at hand. A future in which genes from any kind of cell (prokaryote or eukaryote, e.g. human) might be moved at will from one cell to another was suddenly more realistic than it had ever been.
Spend ~ 1 hour reading the chapter and answering the following questions:

  1. From pages 16-28: Find 1 sentence related to Professor Beckwith's development as a scientist that struck you for its relevance, its surprising nature, its humor, etc. Give the quote and explain its relevance to you.
  2. From page 28-end: In plain language describe what is being shown in
    • figure 1 panel A
    • figure 1 panel C
    • figure 1 panel E.
    • figure 2, top and bottom of image where there are squiggles
    • figure 2, middle where there is one thicker line. Be sure to say why the line is thicker.
    • figure 2 vs figure 3. What has been done to the material in figure 2 to make it appear as it does in figure 3.
  3. From page 36: Comment on the quote, "We have no right to pat ourselves on the back." For example think about:
    • Why was it said?
    • Do you agree with the thought?
    • Would you have said it?
    • Is there some emerging technology you are imagining this century might similarly "hold dangers for humanity."
    • Finally, is your thinking affected by the public reaction to the comment ("newspaper headlines warned of the imminent creation of new Frankenstein's monsters.")?

Upload your answers to the following questions into your personal design portfolios that are here

Very last thing (promise) is to come to class on Tuesday with a question to ask Prof. Beckwith. Write this question on a 3x5 card or piece of paper so we can collect them and give him some choice of topics to address. Thanks!!