20.109(S13): TA notes for module 1: Difference between revisions
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'''Materials required:''' | '''Materials required:''' | ||
*Bird samples! At 100 μL each in a '''2 mL''' tube | *Bird samples! At 100 μL each in a '''2 mL''' tube | ||
**Use filter pipet tip to transfer -- consult Agi about which samples to prepare | |||
*Aliquots of components from QiaAMP stool kit (see GoogleDoc) | *Aliquots of components from QiaAMP stool kit (see GoogleDoc) | ||
**Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early | **Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early | ||
**Some aliquots may be approximate (use your judgment). For example, it will be easier to pipet approximately 0.5 mL into each tube using a serological pipet than to pipet exactly 0.46 mL eight times per day, times several reagents! | |||
*InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one | *InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one | ||
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*Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C | *Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C | ||
*Either set up second heat block at 56 °C or turn on the small oven (may be easiest) | *Either set up second heat block at 56 °C or turn on the small oven (may be easiest) | ||
'''After Lab:''' | '''After Lab:''' | ||
*Store extraction samples at -20 °C until next time | |||
'''How it went:''' | '''How it went:''' | ||
Revision as of 07:45, 10 February 2013
Coming soon!
General notes
See also GoogleDoc for aliquoting amounts.
Scheme:
Key preparation:
Day-by-day
Day 1
Materials required:
Day of Lab (R/F):
After Lab:
How it went: Pre-lab ran longer than it should have, leaving students with just over two hours to complete their designs -- not enough time for most.
Day 2
Materials required:
- Bird samples! At 100 μL each in a 2 mL tube
- Use filter pipet tip to transfer -- consult Agi about which samples to prepare
- Aliquots of components from QiaAMP stool kit (see GoogleDoc)
- Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early
- Some aliquots may be approximate (use your judgment). For example, it will be easier to pipet approximately 0.5 mL into each tube using a serological pipet than to pipet exactly 0.46 mL eight times per day, times several reagents!
- InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one
Day of Lab (R/F):
- Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench
- Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C
- Either set up second heat block at 56 °C or turn on the small oven (may be easiest)
After Lab:
- Store extraction samples at -20 °C until next time
How it went:
