Difference between revisions of "20.109(S13): Final Project WFRedBlue"

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(Topic and Focus)
(Background)
 
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- Implements boolean logic gates: NAND, XOR, and N-IMPLY, half-subtractor, half-adder
 
- Implements boolean logic gates: NAND, XOR, and N-IMPLY, half-subtractor, half-adder
 +
 
- Gradual disassembly of transcriptional / translational components exhibited characteristics of simpler logic gates
 
- Gradual disassembly of transcriptional / translational components exhibited characteristics of simpler logic gates
 +
 
- Individual components can be rewired into combinatorial structures (modular)
 
- Individual components can be rewired into combinatorial structures (modular)
 +
 
- Novelty: all components of logic gates contained in one cell (opposed to others which incorporate intercellular signaling)
 
- Novelty: all components of logic gates contained in one cell (opposed to others which incorporate intercellular signaling)
 +
 
- Goal: Perform therapeutic calculations in animal metabolism
 
- Goal: Perform therapeutic calculations in animal metabolism
  

Latest revision as of 08:51, 8 May 2013

W/F Red/Blue Team Final Project

Topic and Focus

Topic: Cellular Programming and Biosensors

Specific focus Using biosensors to refine and amplify cellular logic gates in order to produce appropriate outputs.

Background

1. http://www.nature.com/nature/journal/vaop/ncurrent/full/nature11149.html

- Implements boolean logic gates: NAND, XOR, and N-IMPLY, half-subtractor, half-adder

- Gradual disassembly of transcriptional / translational components exhibited characteristics of simpler logic gates

- Individual components can be rewired into combinatorial structures (modular)

- Novelty: all components of logic gates contained in one cell (opposed to others which incorporate intercellular signaling)

- Goal: Perform therapeutic calculations in animal metabolism

2. Synthetic circuits integrating logic and memory in living cells[1]

In order to generate complex, state-dependent responses, it is necessary to be able to incorporate logic functions and memory stores. In this study, researchers used recombinases to create Boolean logic functions and DNA-encoded memory on live E. Coli cells. The purpose of this is to be able to create cellular networks capable of producing certain responses given input signals. Furthermore, DNA storage could present a new way of long-term storage, due to the cells self propagation and the stability of DNA.

Siuti, P., Yazbek, J., & Lu, T. K. (2013). Synthetic circuits integrating logic and memory in living cells. Nature Biotechnology, 1–6. doi:10.1038/nbt.2510

3. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564264/

Details/Methods

- Fluorescence Assay - Cell Viability Assay


Predicted Outcomes

- Engineered tunability of logic gate sensitivity to threshold concentrations of an input substance


Necessary Resources: