20.109(S13):PCR and paper discussion (Day3): Difference between revisions
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==Protocols== | ==Protocols== | ||
===Part 1: Prepare PCR to detect bacterial 16S=== | |||
<font color=red> need to do microsporidia PCR on separate day because the Ta etc. is different --> need to find notes on where I planned to put that. possibly Day 2? primers should have arrived by then. then intro structure also needs to change</font color> | |||
===Part 2: WAC Session=== | |||
Today you will hear a lecture on preparing your journal club presentations. | |||
===Part 3: Journal article discussion=== | |||
Scientific papers are dense and often time-consuming to read and understand, but with practice, you will find strategies that improve your comprehension efficiency. Here's one tip to get you started: when reading newly reported results, be sure to refer to the associated figures frequently, because visual information is often easier to take in than purely verbal descriptions. | |||
====Technical Background==== | |||
====Discussion Topics==== | |||
=====Writing===== | |||
As you read the paper by WHOEVER, consider not only its scientific content, but also the authors' writing style (perhaps not all on one read!). Sketch out answers to the questions below (right on the paper if you wish). Your answers will not be collected, but you may be called on in discussion to share your ideas. | |||
=====Content===== | |||
<font color=red>Probably changing to assigning these in advance and doing slightly more formal presentation (single slide prepared) </font color> | |||
When you arrive in lab today, each group will be assigned one of the following topics to present to and discuss with the rest of the class. You should be somewhat familiar with the whole WHOEVER paper by now, but will have some time in-class to refresh your memory and become the resident expert in one of the following areas. | |||
==For next time== | ==For next time== | ||
==Reagent list== | ==Reagent list== | ||
Revision as of 13:52, 6 December 2012
Introduction
PCR background
including why we are using a proofreading polymerase and BSA in this particular situation
maybe gel electrophoresis background also, so don't have to cover it along with everything else next time
Protocols
Part 1: Prepare PCR to detect bacterial 16S
need to do microsporidia PCR on separate day because the Ta etc. is different --> need to find notes on where I planned to put that. possibly Day 2? primers should have arrived by then. then intro structure also needs to change
Part 2: WAC Session
Today you will hear a lecture on preparing your journal club presentations.
Part 3: Journal article discussion
Scientific papers are dense and often time-consuming to read and understand, but with practice, you will find strategies that improve your comprehension efficiency. Here's one tip to get you started: when reading newly reported results, be sure to refer to the associated figures frequently, because visual information is often easier to take in than purely verbal descriptions.
Technical Background
Discussion Topics
Writing
As you read the paper by WHOEVER, consider not only its scientific content, but also the authors' writing style (perhaps not all on one read!). Sketch out answers to the questions below (right on the paper if you wish). Your answers will not be collected, but you may be called on in discussion to share your ideas.
Content
Probably changing to assigning these in advance and doing slightly more formal presentation (single slide prepared)
When you arrive in lab today, each group will be assigned one of the following topics to present to and discuss with the rest of the class. You should be somewhat familiar with the whole WHOEVER paper by now, but will have some time in-class to refresh your memory and become the resident expert in one of the following areas.
