Difference between revisions of "20.109(S13):DNA cloning (Day4)"

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==Reagent list==
 
==Reagent list==
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Revision as of 13:05, 6 December 2012


20.109(S13): Laboratory Fundamentals of Biological Engineering

S13 109-OWW-frontpage.jpg

Home        Schedule Spring 2013        Assignments       
DNA Engineering        Protein Engineering        Cell Engineering              

Introduction

background on this cloning system -- vector arms and strain

transformation

Protocols

Part 1: Purify 16S PCR band from gel

Part 2: Ligate purified product to vector arms

Part 3: Transform ligated product into cloning strain

For next time

Reagent list

write something here or not accessible to edit