20.109(S11): Spring 2011 schedule: Difference between revisions
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| [[20.109(S11):Lab tour | Orientation]] | | [[20.109(S11):Lab tour | Orientation]] | ||
| | | *[[Media:20.109 OrientLect(S11).pdf | Introductory Lecture (PDF)]] <br>*[[Media:S11_Orient_Pre-Lab_ANS.pdf | Intro Pre-Lab, T/R]] | ||
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| colspan="7" | | | colspan="7" | | ||
Revision as of 10:29, 1 February 2011
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| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 1 | ANS | Orientation | * Introductory Lecture (PDF) * Intro Pre-Lab, T/R | |||
| 1 | 1 | Thur Feb 3 | JN | CT | Amplify aptamer-encoding DNA | |
| 1 | 2 | Tue Feb 8 | JN | CT | Purify aptamer-encoding DNA | |
| 1 | 3 | Thur Feb 10 | JN | CT | Prepare RNA by IVT | |
| 1 | 4 | Tue Feb 15 | JN | CT | Purify RNA and run affinity column | |
| 1 | 5 | Thur Feb 17 | JN | CT | RNA to DNA by RT-PCR | |
| President's Day Feb 21 | No lab Feb 22 or Feb 23 | |||||
|---|---|---|---|---|---|---|
| 1 | 6 | Thur Feb 24 | JN | CT | Post-selection IVT and journal club | |
| 1 | 7 | Tue Mar 1 | JN | CT | Aptamer binding assay | |
| 1 | 8 | Thur Mar 3 | JN | CT | Journal club | |
| 2 | 1 | Tue Mar 8 | RW | AR | Explore model system | Module 1 Draft Due |
| 2 | 2 | Thur Mar 10 | RW | AR | Complete DNA design | |
| 2 | 3 | Tue Mar 15 | RW | AR | Prepare DNA for cloning | |
| 2 | 4 | Thur Mar 17 | RW | AR | Ligate DNA and transform bacteria | |
| Spring Break Mar 21-25 | No lab all week | |||||
| 2 | 5 | Tue Mar 29 | RW | AR | Prepare candidate clones in model cell strain | |
| 2 | 6 | Thur Mar 31 | RW | AR | Evaluate DNA and choose clone | Module 1 Final Report Due |
| 2 | 7 | Tue Apr 5 | RW | AR | Assay modified model system | |
| 2 | 8 | Thur Apr 7 | RW | AR | Wrap-up analysis and modeling | |
| 3 | 1 | Tues Apr 12 | ANS | HHH | Start-up biomaterials engineering | Module 2 Draft Due |
| 3 | 2 | Thur Apr 14 | ANS | HHH | Initiate cell culture | |
| Patriot's Day April 18-19 | No lab Apr 19 or Apr 20 | |||||
| 3 | 3 | Thur Apr 21 | ANS | HHH | Testing cell viability | |
| 3 | 4 | Tue Apr 26 | ANS | HHH | Preparing cells for analysis | |
| 3 | 5 | Thur Apr 28 | ANS | HHH | Initiating transcript and protein assays | Module 2 Final Report Due |
| 3 | 6 | Tue May 3 | ANS | HHH] | Transcript-level analysis | |
| 3 | 7 | Thur May 5 | ANS | HHH | Protein-level and wrap-up analysis | Module 3 Summary Due |
| 3 | 8 | Tue May 10 | ANS | HHH | Student presentations | |
| Thur May 12 | No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD | |||||
