Difference between revisions of "20.109(F12): Quick Facts"

From OpenWetWare
(Taq Polymerase)
(Taq Polymerase)
Line 3: Line 3:
 
* Taq has an error rate of about 1 in 9000. Reference: [http://www.ncbi.nlm.nih.gov/pubmed/2847780 an article in the journal Biochemistry, quick info can be found in the abstract].
 
* Taq has an error rate of about 1 in 9000. Reference: [http://www.ncbi.nlm.nih.gov/pubmed/2847780 an article in the journal Biochemistry, quick info can be found in the abstract].
 
** So would you use Taq for cloning purposes?
 
** So would you use Taq for cloning purposes?
** What is the probability of finding a mutation in a GFP gene?
+
** What is the probability of finding a mutation in a GFP gene PCR-ed up using Taq polymerase?

Revision as of 12:09, 8 September 2012

DNA Polymerases

Taq Polymerase