20.109(F12): Quick Facts: Difference between revisions
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* Taq has an error rate of about 1 in 9000. Reference: [http://www.ncbi.nlm.nih.gov/pubmed/2847780 an article in the journal Biochemistry, quick info can be found in the abstract]. | * Taq has an error rate of about 1 in 9000. Reference: [http://www.ncbi.nlm.nih.gov/pubmed/2847780 an article in the journal Biochemistry, quick info can be found in the abstract]. | ||
** So would you use Taq for cloning purposes? | ** So would you use Taq for cloning purposes? | ||
** What is the probability of finding a mutation in a GFP gene? | ** What is the probability of finding a mutation in a GFP gene PCR-ed up using Taq polymerase? | ||
Revision as of 12:09, 8 September 2012
DNA Polymerases
Taq Polymerase
- Taq has an error rate of about 1 in 9000. Reference: an article in the journal Biochemistry, quick info can be found in the abstract.
- So would you use Taq for cloning purposes?
- What is the probability of finding a mutation in a GFP gene PCR-ed up using Taq polymerase?
