20.109(F08):Module 3

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20.109(F08): Laboratory Fundamentals of Biological Engineering


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Module 3

Instructors: Angela Belcher, Natalie Kuldell and Agi Stachowiak

TA:Luis Alvarez

“Invention” is a wonderful word, derived from words meaning “scheme” and “a finding out.” Inventors draw on materials provided by the natural world, refining and combining them in insightful ways, to make something useful. In this experimental module we will invent materials by manipulating biological systems, namely the bacteriophage M13. We will use a very slightly modified phage to build Iridium nanowires and then we'll let the phage themselves do the building, making an electrochromic device that's both fun and potentially useful. Drawing on the rich stockroom of biological elements and a good but incomplete understanding of their behavior, we’ll hope to invent some novel materials with real-world applications.

TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam

Day 1: Phage growth for materials
Day 2: Phage nanowires
Day 3: Journal Club II
Day 4: Phage by design
Day 5: Phage by design, pt 2
Day 6: ECD assembly
Day 7: Oral presentations
direct link to experimental variations page

Notes for Teaching Faculty

TA notes, mod 3