Difference between revisions of "-80 Glycerol Stocks"

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(Protocol)
 
(One intermediate revision by the same user not shown)
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===Protocol===
 
===Protocol===
*add 1 equivalent of 50% glycerol to overnight  stock
+
*note: only make '''ONE''' -80 tube for each sample
*add 300μL mixture to each barcode tube
+
*mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
 
*scan each tube on Simple Scan 1000 (on the computer next to the robot)
 
*scan each tube on Simple Scan 1000 (on the computer next to the robot)
***enter data, and click save
+
**enter data, and click save
 
*log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
 
*log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
 
*store in -80
 
*store in -80

Latest revision as of 10:53, 9 June 2010

Protocol

  • note: only make ONE -80 tube for each sample
  • mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
  • scan each tube on Simple Scan 1000 (on the computer next to the robot)
    • enter data, and click save
  • log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
  • store in -80