Difference between revisions of "-80 Glycerol Stocks"

From OpenWetWare
Jump to: navigation, search
(New page: ===Protocol=== *add 1 equivalent of 50% glycerol to overnight stock *add 300μL mixture to each barcode tube *scan each tube on Simple Scan 1000 (on the computer next to the robot) -enter...)
 
(Protocol)
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
 
===Protocol===
 
===Protocol===
*add 1 equivalent of 50% glycerol to overnight  stock
+
*note: only make '''ONE''' -80 tube for each sample
*add 300μL mixture to each barcode tube
+
*mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
 
*scan each tube on Simple Scan 1000 (on the computer next to the robot)
 
*scan each tube on Simple Scan 1000 (on the computer next to the robot)
-enter data, and click save
+
**enter data, and click save
*log position and data into [https://spreadsheets.google.com/ccc?key=0AllHPRPS0Xc2dG1xZV9ud2hPTzVRTXhoTG5YWjh5SXc&hl=en#gid=2 google docs file]
+
*log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
 
*store in -80
 
*store in -80

Latest revision as of 09:53, 9 June 2010

Protocol

  • note: only make ONE -80 tube for each sample
  • mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
  • scan each tube on Simple Scan 1000 (on the computer next to the robot)
    • enter data, and click save
  • log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
  • store in -80