OhioMod2013:Results
From OpenWetWare
Results
Nanoparticle Characterizatione
HPLC
Show A280 peaks, determine rate of DNA encapsulation vs free DNA.
Zetasizer
Determine size and zeta potential of particles.
TEM
Take direct measurements of ~100 particles. Determine consistency of size.
Dissolution
Perform titration of nanoparticles until dissolution of nanoparticles. Run gel for DNA origami vs. free DNA after dissolution. Is origami intact?
Serum Assay
Apply DNA encapsulated particles vs free DNA origami vs PEGylated particles to bovine serum. Measure intact origami after period of time.
Cytotoxicity
Perform MTT assay.