10/24/12
- Check results ofr section 1
- Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes
Check results (spot check)
Group 1 1. ctrl 2. ctrl 3. plasmid 4. plasmid 5. plasmid 6. none 7. none 8. none
Group 2 1. ctrl 2. ctrl 3. none 4. none 5. none 6. plasmid 7. plasmid 8. plasmid
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Prep for Week 2, Wednesday (Sections 2, 3)
- Assign biomarker status to fictional patients on Patient Assignment Table (Google doc)
- Put into baggies: 1 marker, 1 PCR tube rack, 1 trimmed plastic bulb pipette (to be fitted into disposable sterile pipette tips, provided by Garcia)
- Master Mixes
- DONE: Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total
- To do: DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group)
DNA/primer mixes: Make ~1/3 "patients" positive for marker
Section |
Groups |
Total Patients |
Pos ctrls |
Pos patients x3 reps |
TOTAL (+)'s |
Neg ctrls |
Neg patients x3 reps |
TOTAL (-)'s
|
Thurs 9:30 am |
17 |
34 |
17 |
36 |
53 |
17 |
66 |
83
|
Thurs 1:30 pm |
17 |
34 |
17 |
36 |
53 |
17 |
66 |
83
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Per Section!
Reagent |
(+) |
Mix+2 (x55) |
(-) |
Mix+2 (x85)
|
Primer 1 (P0001) |
1.0 |
55.0 |
1.0 |
85.0
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Primer 2 (P0002) |
1.0 |
55.0 |
1.0 |
85.0
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Kozak plasmid (54 ng/μL) |
0.2 |
11 |
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dH2O |
47.8 |
2629.0 |
48.0 |
4080
|
|
50.0 |
2750 |
50.0 |
4250
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