Smolke:Protocols/Gel extraction

Home        Contact        Internal        Protocols        Lab Members        Publications        Research       

Comb size Single wells from the smallest comb (15 wells per row) can hold up to 20 ul Single wells from un-taped medium comb (8 wells per row) can hold up to 50 ul Agarose density Above 3 kb: 0.8% 1-3 kb: 1% 250-1000 bp: 1.5% Below 250 bp: 2% 75V for 45 min, or 85V for 35 min Minimizing the amount of agarose in the cutout band seems to improve yields Notes I clean the razor blade between each use with a Kimwipe and do not change blades until it is dull or known to be contaminated. I have never had cross-contamination in my gel extraction doing it this way. --YC Using the nano-drop to determine DNA concentration from a gel extraction is inaccurate. If you really need a concentration, run a small sample of the eluent on a gel and determine the concentration by comparing the brightness to that of the ladder at known concentration.