Strain or plasmid | Relevant properties or genotype | <NOBR
> Source</NOBR
> |
|---|
|
| <NOBR
> Strains</NOBR
> | <NOBR
></NOBR
> | <NOBR
></NOBR
> |
E. coli DH5<IMG
SRC="/giflibrary/12/alpha.gif"
BORDER="0"> | F-, <IMG
SRC="/giflibrary/12/phgr.gif"
BORDER="0"
ALIGN="ABSBOTTOM">80d, lacZ<IMG
SRC="/giflibrary/12/Delta.gif"
BORDER="0">M15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, <IMG
SRC="/giflibrary/12/Delta.gif"
BORDER="0">(lacZYA-argF)U169 | <NOBR
> [a]</NOBR
> |
E. coli M15 [pREP4] | F-, lacZ<IMG
SRC="/giflibrary/12/Delta.gif"
BORDER="0">M15, thi-1, lac-, mtl-, recA+ , KmR | <NOBR
> [a]</NOBR
> |
Pseudomonas putida KT2440 | type strain | <NOBR
> [b]</NOBR
> |
Synechocystis sp. PCC 6803 | type strain | <NOBR
> [c]</NOBR
> |
| <NOBR
> Plasmids</NOBR
> | <NOBR
></NOBR
> | <NOBR
></NOBR
> |
| <NOBR
> pCAR16</NOBR
> | <IMG
SRC="/giflibrary/12/beta.gif"
BORDER="0"
ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR | <NOBR
> [<A
HREF="#BIB27"
>27</A
>]</NOBR
> |
| <NOBR
> pJF119EH</NOBR
> | cloning vector, tac-promoter, IPTG inducible, AmpR | <NOBR
> [<A
HREF="#BIB25"
>25</A
>]</NOBR
> |
| <NOBR
> pJF119<IMG
SRC="/giflibrary/12/Delta.gif"
BORDER="0">N</NOBR
> | cloning vector, RBS, tac-promoter, IPTG inducible, AmpR | this study |
| <NOBR
> pAW229</NOBR
> | cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR | <NOBR
> [<A
HREF="#BIB26"
>26</A
>]</NOBR
> |
| <NOBR
> pQE31-VTE1</NOBR
> | tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR | <NOBR
> [<A
HREF="#BIB18A"
>18a</A
>]</NOBR
> |
| <NOBR
> pCAS2JF</NOBR
> | 1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH | this study |
| <NOBR
> pCAS8</NOBR
> | 1.1 kb NdeI/BamHI PCR fragment ggh in pJF119<IMG
SRC="/giflibrary/12/Delta.gif"
BORDER="0">N | this study |
| <NOBR
> pCAS11</NOBR
> | 0.9 kb BglII/BamHI PCR fragment crtE in pCAS8 | this study |
| <NOBR
> pCAS12</NOBR
> | 1.0 kb BglII/BamHI PCR fragment hpt in pCAS11 | this study |
| <NOBR
> pCAS15</NOBR
> | 1.2 kb NdeI/BamHI PCR fragment hpd in pAW229 | this study |
| <NOBR
> pCAS18</NOBR
> | 1.2 kb BglII/BamHI PCR fragment hpd in pCAS11 | this study |
| <NOBR
> pCAS19</NOBR
> | 1.0 kb NdeI/BamHI PCR fragment hpt in pAW229 | this study |
| <NOBR
> pCAS23</NOBR
> | 1.0 kb NdeI/BamHI PCR fragment hpt in pJF119<IMG
SRC="/giflibrary/12/Delta.gif"
BORDER="0">N | this study |
| <NOBR
> pCAS24</NOBR
> | 0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23 | this study |
| <NOBR
> pCAS27</NOBR
> | 1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229 | this study |
| <NOBR
> pCAS29</NOBR
> | 1.2 kb BglII/BamHI PCR fragment hpd in pCAS18 | this study |
| <NOBR
> pCAS30</NOBR
> | 0.9 kb NdeI/BamHI PCR fragment crtE in pJF119<IMG
SRC="/giflibrary/12/Delta.gif"
BORDER="0">N | this study |
| <NOBR
> pCAS37</NOBR
> | 1.2 kb BglII/BamHI PCR fragment hpd in pCAS24 | this study |
| <NOBR
> pCAS47</NOBR
> | 1.3 kb BglII PCR fragment vte1 in pCAS29 | this study |
