User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/03
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June 3, 2013Absorbance and Fluorescence DataFinish preparing thiol DNA, AuNP and ThT sample<br.> 2[ThT]:1[DNA]→ 2.2245μM ThT<br.> 10% of 250μL=25μL (within 10% allows for accurate concentration comparison)<br.> Total solution volume: 275μL<br.> (2.2245×10-6M)(275×10-6L)=6.11738×10-10moles ThT<br.> (6.11738×10-10moles)/(25×10-6L)=24.5μM ThT<br.> <br.> To make 24.5μM ThT:<br.> (120μM)V=(24.5μM)(25μL)<br.> V=5.1μL in 19.9μL PBS buffer<br.> <br.> Add 25μL of 24.5μM ThT to 250μL DNA/AuNP solution<br.> New concentrations:
<br.> <br.> <br.> <br.> <br.> <br.> <br.> NotesSamples containing Molecular Beacon excited at 532nm<br.> Samples containing ThT excited at 440nm<br.> Sample Preparation for FCS and Calculations |