Yu:Dialysis

SpectraPor Membrane Dialysis

1. Cut an appropriate length of dialysis tubing. You will need 1cm for tying, plus 1cm tubing per 1mL.

2. Soak in a large volume of distilled H2O for 30 minutes

3. Soak in a large volume of 2% Sodium Bicarbonate (w/v), 1mM EDTA at 60°C for 30 minutes

4. Rinse thoroughly in a large volume of distilled H2O.

5. Carefully tie off one end of the tubing with a double-knot.

6. Add solution to be dialyzed by pipetting.

7. Clamp off the open end with a plastic floating clamp.

8. Suspend in the dialysis solution in a large beaker or graduated cylinder, depending on the length of the dialysis tubing.

9. Change dialysis solution as needed.

10. When dialysis is complete, carefully pinch off the tubing under the clamp. Remove the clamp carefully - note that the tubing will break where it was clamped if you are not supporting the tubing.

11. Carefully decant the dialyzed solution by pipetting or by pouring.