Haynes Lab:Notebook/Engineering PC-TFs/2013/01/25: Difference between revisions
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* 95°C, 3 min. | * 95°C, 3 min. | ||
* [95°C, 30 sec.; 57°C, 30 sec.; 72°C, 1 min.] x35 | * [95°C, 30 sec.; 57°C, 30 sec.; 72°C, 1 min.] x35 | ||
* 72°C, 3 min. | * 72°C, 3 min. | ||
* 4°C, ∞ | * 4°C, ∞ | ||
Purification | |||
* Clean with Zymo DNA c&c; elute w/ 20 μL dH<sub>2</sub>O | |||
* Measure [DNA] | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Sample | |||
| bgcolor=#cfcfcf | OD 260 | |||
| bgcolor=#cfcfcf | 260/280 | |||
| bgcolor=#cfcfcf | ng/μL | |||
|- | |||
| 1. pSB1A3 || ### || ### || ### | |||
|- | |||
| 2. ### || ### || ### || ### | |||
|- | |||
| 3. ### || ### || ### || ### | |||
|} | |||
Revision as of 13:41, 25 January 2013
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||
01/25/13
PCR
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