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| ==Overview==
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| Replace this sentence with a brief description of the protocol and its goal.
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| ==Materials==
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| For a 50 μL PCR reaction:
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| * 35 μL H<sub>2</sub>O
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| * 5 μL 10X PCR buffer
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| * 5 μL 2mM dNTPs (each)
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| * 1.5 μL 50mM MgCl<sub>2</sub>
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| * 1 μL 50μM sense primer
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| * 1 μL 50μM antisense primer
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| * 1 μL 5nM DNA template
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| * 0.5 μL TAQ DNA polyermerase
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| ==Procedure==
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| # In a PCR tube, mix the components on ice in the order they are listed above.
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| # Perform thermocycling program
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| ## 95 °C 5 min
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| ## 95 °C 30 s
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| ## T<sub>H</sub> 30 s
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| ## 72 °C 1 min for each 1 kb PCR product
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| ## Repeat steps 2-4 a total of 12-36 times (24 is standard).
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| ## 72 °C 5 min
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| ## 12 °C hold
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| ==Notes==
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| Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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| #List troubleshooting tips here.
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| #You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
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| #Anecdotal observations that might be of use to others can also be posted here.
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| Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
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| ==References==
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| '''Relevant papers and books'''
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| <!-- If this protocol has papers or books associated with it, list those references here.-->
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| <!-- See the [[OpenWetWare:Biblio]] page for more information, but this format doesn't work currently.
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| <biblio>
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| #Goldbeter-PNAS-1981 pmid=6947258
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| #Jacob-JMB-1961 pmid=13718526
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| #Ptashne-Genetic-Switch isbn=0879697164
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| </biblio>-->
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| <!-- Try the [[Template:FormatRef|FormatRef template]]-->
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| #{{FormatRef|Goldbeter, A and Koshland, DE|1981| |Proc Natl Acad Sci U S A 78(11)|6840-4| }} PMID 6947258
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| #{{FormatRef|Jacob, F and Monod, J J|1961| |Mol Biol 3(3)|318-56| }} PMID 13718526
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| #{{FormatRef|Ptashne, M|2004|Genetic Switch: Phage Lambda Revisited| | |Cold Spring Harbor Laboratory Press}} ISBN 0879697164
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| ==Contact==
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| *Who has experience with this protocol?
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| or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].
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| <!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
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| <!-- Move the relevant categories above this line to tag your protocol with the label
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| [[Category:Protocol]]
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| [[Category:Needs attention]]
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| [[Category:In vitro]]
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| [[Category:In vivo]]
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| [[Category:In silico]]
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| [[Category:DNA]]
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| [[Category:RNA]]
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| [[Category:Protein]]
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| [[Category:Chemical]]
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| [[Category:Escherichia coli]]
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| [[Category:Yeast]]
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