Frankel:Force Spectroscopy: Difference between revisions
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< | '''<font align="center" font color=#ffffff font size=8>______ </font>''''''<font align="center" font color=#000000 font size=8>Force Spectroscopy</font>''' | ||
'''<font align="center" font color=#000000 font size=8>Force Spectroscopy</font>''' | |||
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'''<font color=# | |||
'''<font color=#000000 font size=3>Picking up and unfolding a single protein</font>''' | |||
[[Image:Spectroscopy_complete.jpg|350px]] | [[Image:Spectroscopy_complete.jpg|350px]] | ||
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'''<font color=#FFFFFF font size=3>Assembly</font>''' | '''<font color=#FFFFFF font size=3>Assembly</font>''' | ||
'''<font color=#000000 font size=3> | '''<font color=#000000 font size=3>Nano-mechanics of HIV GP160</font>''' | ||
[[Image:GP160mica.png|300px]] | [[Image:GP160mica.png|300px]] | ||
[[Image:GP_160.png|300px]] | |||
'''<font color=#FFFFFF font size=6>GP160mica</font>''' | '''<font color=#FFFFFF font size=6>GP160mica</font>''' | ||
[[Image:Stlow.png|330px]] | [[Image:Stlow.png|330px]] | ||
[[Image:Hislow.png|330px]] | [[Image:Hislow.png|330px]] | ||
---- | ---- | ||
'''<font color=#000000 font size=3 font align="justify"> | '''<font color=#000000 font size=3 font align="justify"> | ||
Typical sawtooth unfolding pattern of a single molecule of the HIV virion protein GP160. Unfolding forces can be extracted from the sawtooth pattern by applying the worm like chain model.</font>''' | |||
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'''<font color=#000000 font size=3> | '''<font color=#000000 font size=3> Unfolding the ECM protein fibronectin </font>''' | ||
[[Image:FNsawt.png|300px]] | [[Image:FNsawt.png|300px]] | ||
[[Image:HisFN.png|300px]] | [[Image:HisFN.png|300px]] | ||
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'''<font align="justify" font size=3> | '''<font align="justify" font size=3> | ||
Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface was 85.1 ± 2.7 pN. | Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface was 85.1 ± 2.7 pN. | ||
Latest revision as of 18:22, 9 January 2013
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'______ 'Force Spectroscopy
Picking up and unfolding a single protein |
Assembly Nano-mechanics of HIV GP160 GP160mica
Typical sawtooth unfolding pattern of a single molecule of the HIV virion protein GP160. Unfolding forces can be extracted from the sawtooth pattern by applying the worm like chain model.
| |
Unfolding the ECM protein fibronectin
Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface was 85.1 ± 2.7 pN. |