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[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel Info''' </font>]]
[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel Info''' </font>]]
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=Protocols=
=Protocols=


'''BioBrick Assembly'''
==DNA Assembly==
* [[Haynes:BioBrick Method short | Assembly of BioBrick Parts: an Overview]]
* [[Haynes:BioBrick Method short | BioBrick Parts Assembly: an Overview]]
* [[Haynes:Making BioBricks | Making Standardized DNA Parts]]
** [[Haynes:Making BioBricks | Making Standardized BioBrick Parts]]
* [[Haynes:Assembly101 | Model Procedure for Assembling Parts: Classic Ligation for Beginners]]
** [[Haynes:Assembly101 | Model Procedure for Assembling BioBrick Parts: Classic Ligation for Beginners]]
* [[Haynes:NewProtocol | New Protocol]]
* [[Haynes:Gibson_Assembly | Gibson Assembly]]


<br>


'''Cell Culture: Bacteria'''
==Cell Culture: Bacteria==
* [[Haynes:ChemComp cells | Chemically competent cell prep]]
* [[Haynes:ChemComp cells | Chemically competent cell prep]]
* [[Haynes:Glycerol Stocks | Glycerol Stocks]] - for long term -80°C storage
* [[Haynes:Glycerol Stocks | Glycerol Stocks]] - for long term -80°C storage
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'''Cell Culture: Mammalian'''
==Cell Culture: Mammalian==
* [[Haynes:MamCultureMedia | Media formulas]] - cell line-specific formulas
* [[Haynes:MamCultureMedia | Media formulas]] - cell line-specific formulas
* [[Haynes:TransfectionPlasmid_Lipo | Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine


 
==Protein==
'''Protein'''
* [[Haynes:Bradford | Bradford assay]] - measure protein concentration
* [[Haynes:Bradford | Bradford assay]] - measure protein concentration




'''Real Time Quantitative PCR'''
==Real Time Quantitative PCR==
* [[Haynes:UPLassay | Universal Probe Library (UPL) assay]]: for the Roche Light Cycler 480
* [[Haynes:UPLassay | Universal Probe Library (UPL) assay]]: for the Roche Light Cycler 480




'''Resources at OpenWetWare'''
==Resources at OpenWetWare==
* [http://openwetware.org/wiki/E._coli_genotypes E. coli Strains]  
* [http://openwetware.org/wiki/E._coli_genotypes E. coli Strains]  
* [http://openwetware.org/wiki/Materials Materials]: buffer formulas; specific information on commonly used reagents (''e.g.'', LB broth, ampicillin, restriction enzymes, etc.)
* [http://openwetware.org/wiki/Materials Materials]: buffer formulas; specific information on commonly used reagents (''e.g.'', LB broth, ampicillin, restriction enzymes, etc.)




'''Software Guides'''
==Software Guides==
* [[Haynes:Jmol_Guide | Jmol commands: controlling PDB structure displays]]
* [[Haynes:Jmol_Guide | Jmol commands: controlling PDB structure displays]]


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'''Bromo-Blue/X-cyanol Loading Buffer, 10X''' {{hide|  
'''Bromo-Blue/X-cyanol Loading Buffer, 20X''' {{hide|  
Formula: [30% glycerol; 12.5 mg/mL bromophenol blue; 12.5 mg/mL xylene cyanol]<br>
Formula: [60% glycerol; 12.5 mg/mL bromophenol blue; 12.5 mg/mL xylene cyanol]<br>
Volume: 20 mL
Volume: 20 mL
* 50% glycerol, 12 mL
* 100% glycerol, 12 mL
* Bromophenol blue, 250 mg
* Bromophenol blue, 250 mg
* Xylene cyanol, 250 mg
* Xylene cyanol, 250 mg


Bring up to total volume with dH<sub>2</sub>O.
Start with 18 mL dH<sub>2</sub>O. Add in glycerol 6 mL at a time and mix by pipetting up and down several times (it is very viscous). Add dyes.
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Bring up to total volume with dH<sub>2</sub>O. Store at 4°C. Add 100x protease inhibitor cocktail* (1:100) immediately before use.
Bring up to total volume with dH<sub>2</sub>O. Store at 4°C. Add 100x protease inhibitor cocktail* (1:100) immediately before use.
}}
'''DNA Ladder mix, Gene Ruler 1 kb Plus''' {{hide|
Formula: [0.5 ng/ 10 μL Fermentas Gene Ruler 1 kb Plus; 1x loading dye]<br>
Volume: 1000 μL
* Gene Ruler plus (0.5 ng/μL), 100 μL
* 20x loading dye, 50 μL
* dH<sub>2</sub>O, 850 μL
Add dH<sub>2</sub>O directly to the supplier's tube (containing 100 μL of ladder). Add the 20x loading dye. Mix. Aliquot 200 μL portions into five 1.5 mL tubes. Store at -20°C.
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'''Mammalian Cell Media''' {{hide|  
'''Mammalian Cell Media''' {{hide|  
Volume: 500 mL
Volume: 500 mL
* [[Haynes:MamCellMedia | Appropriate incomplete medium]], 500 mL
* [[Haynes:MamCultureMedia | Appropriate incomplete medium]], 500 mL
* Fetal bovine serum (FBS), 50 mL
* Fetal bovine serum (FBS), 50 mL
* Penicillin/ streptomycin (pen-strep), 5 mL
* Penicillin/ streptomycin (pen-strep), 5 mL
* [[Haynes:MamCellMedia | Appropriate antibiotics]], depending upon formula
* [[Haynes:MamCultureMedia | Appropriate antibiotics]], depending upon formula


Add FBS and pen-strep directly to the incomplete medium in the original stock bottle. Cap and invert to mix. Filter sterilize using a vacuum filtration unit.
Add FBS and pen-strep directly to the incomplete medium in the original stock bottle. Cap and invert to mix. Filter sterilize using a vacuum filtration unit.
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