IGEM:IMPERIAL/2006/LabCalendar/2006-8-29: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 7: | Line 7: | ||
===To Do:=== | ===To Do:=== | ||
*Culture up the ligated parts (J37022 & J37025) for miniprep (to check whether ligations from Monday | |||
*Culture up the ligated parts (J37022 & J37025) for | |||
were successful) from plates in incubator | were successful) from plates in incubator | ||
*Make frozen stock of 4G(S01656) and J37018 | *Make frozen stock of 4G(S01656) and J37018 | ||
*Make more 50 times TAE | *Make more 50 times TAE | ||
*Culture for testing: T9002, J37015, J37020 | |||
*Culture for testing: T9002, J37015, J37020 | |||
*To order: LB medium (or make up ourselves & send to autoclave) & make some LB Amp to have in stock | *To order: LB medium (or make up ourselves & send to autoclave) & make some LB Amp to have in stock | ||
Autoclaved flasks (and cuvettes?) | Autoclaved flasks (and cuvettes?) | ||
Revision as of 11:22, 29 August 2006
To Do:
*Culture up the ligated parts (J37022 & J37025) for miniprep (to check whether ligations from Monday
were successful) from plates in incubator
*Make frozen stock of 4G(S01656) and J37018
*Make more 50 times TAE
*Culture for testing: T9002, J37015, J37020
*To order: LB medium (or make up ourselves & send to autoclave) & make some LB Amp to have in stock
Autoclaved flasks (and cuvettes?)
Need more eppendorfs
More LB Amp plates ? (How many more do we need for our remaining time - there are about 10 left)
*Gels from the weekend need to be analysed: Were J37018 & J37024 ligations successful ?
LoxP PCR
- Run once more, this time with DMSO, and a larger amount of maxiprepped DNA
