Biomod/2012/Tohoku/Team Sendai: Difference between revisions
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<h1 | <h1 align="center";><!--Team Sendai--><img src="http://openwetware.org/images/c/c2/Title-kai.png" ></h1> | ||
</div> | </div> | ||
< | <h1>Abstract</h1> | ||
<p> | |||
All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.</br> | |||
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br> | |||
Here, we decided to create a novel device with such a function. </br> | |||
We have designed <strong>a cylindrical injector/extractor device made of DNA origami</strong> (we named this device: <strong>CELL-GATE</strong>). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br> | |||
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br> | |||
</p> | |||
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< | <div id="pop"> | ||
<h2><!--Abstract-->Project Image</h2> | |||
<p> | <p> | ||
<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br> | |||
<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.</br> | |||
<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br> | |||
<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane. | |||
</p> | </p> | ||
<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px" usemap="#D-Heart"> | |||
<map name="D-Heart"> | |||
<area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter"> | |||
<area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate"> | |||
<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE" alt="Membarane"> | |||
</map> | |||
Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture! | |||
</div> | |||
<div id="pop"> | <div id="pop"> | ||
<h2>Movie</h2> | <h2>Movie</h2> | ||
<p> | |||
<p> | </br> | ||
<iframe width="420" height="315" src="http://www.youtube.com/embed/8OB8ytltxwY" frameborder="0" allowfullscreen></iframe> | |||
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Latest revision as of 19:43, 27 October 2012
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<body> <div id="Container"> <!-- Menu --> <ul id="menu"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai ">Home</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea ">Project</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Design">Design</a> </li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Simulation">Simulation</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Experiment ">Experiment</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Future Application">Future Application</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Diary">Diary</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Team ">Team</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/Tohoku/Team Sendai/header"></a> </li>
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</body> </div> </html> <html> <div id="Container"> <!—二段組み本文 --> <div id="Header"> <h1 align="center";><!--Team Sendai--><img src="http://openwetware.org/images/c/c2/Title-kai.png" ></h1> </div>
<h1>Abstract</h1> <p> All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.</br> A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br> Here, we decided to create a novel device with such a function. </br> We have designed <strong>a cylindrical injector/extractor device made of DNA origami</strong> (we named this device: <strong>CELL-GATE</strong>). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br> We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br> </p>
<div id="pop"> <h2><!--Abstract-->Project Image</h2> <p> <strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br> <a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.</br> <a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br> <a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane. </p>
<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px" usemap="#D-Heart"> <map name="D-Heart"> <area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter"> <area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate"> <area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE" alt="Membarane"> </map> Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture! </div>
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<h2>Movie</h2>
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