Biomod/2012/UT/Nanowranglers/Results: Difference between revisions
| Line 7: | Line 7: | ||
Buffer: TMgK | Buffer: TMgK | ||
[[Image:8-13-2012TMgKgel.jpeg|350px]] | [[Image:8-13-2012TMgKgel.jpeg|350px]] | ||
[[Image:8-13-2012TMgKtable.jpeg|350px]] | [[Image:8-13-2012TMgKtable.jpeg|350px]] | ||
Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, but this decreases noticeably in the absence of the initiator. From the presence of excess lines in both lanes that contain FD,h, it is clear that there are impurities in FD,h, which will require the test to be run again after the sample is purified. | Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, but this decreases noticeably in the absence of the initiator. From the presence of excess lines in both lanes that contain FD,h, it is clear that there are impurities in FD,h, which will require the test to be run again after the sample is purified. | ||
| Line 20: | Line 16: | ||
Buffer: TMgK | Buffer: TMgK | ||
[[Image:8-14-2012TMgKgel.jpeg|350px]] | [[Image:8-14-2012TMgKgel.jpeg|350px]] | ||
[[Image:8-14-2012TMgKtable.jpeg|350px]] | [[Image:8-14-2012TMgKtable.jpeg|350px]] | ||
Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, regardless of whether the initiator is present. While this trend does decrease slightly when temperature of incubation is decreased and concentration of FA,h is increased, the change is not dramatic enough to make these hairpins viable as fuels in this buffer. | Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, regardless of whether the initiator is present. While this trend does decrease slightly when temperature of incubation is decreased and concentration of FA,h is increased, the change is not dramatic enough to make these hairpins viable as fuels in this buffer. | ||
| Line 33: | Line 25: | ||
Buffer: TNaK | Buffer: TNaK | ||
[[Image:8-14-2012TNaKgel.jpeg|350px]] | [[Image:8-14-2012TNaKgel.jpeg|350px]] | ||
[[Image:8-14-2012TNaKtable.jpeg|350px]] | [[Image:8-14-2012TNaKtable.jpeg|350px]] | ||
Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, regardless of whether the initiator is present. There is no noticeable change in this trend when temperature of incubation is decreased or concentration of FA,h is increased. Therefore, these hairpins are not viable as fuels in this buffer. | Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, regardless of whether the initiator is present. There is no noticeable change in this trend when temperature of incubation is decreased or concentration of FA,h is increased. Therefore, these hairpins are not viable as fuels in this buffer. | ||
Revision as of 00:41, 27 October 2012
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<img src="http://openwetware.org/images/5/59/NanoWranglers.png"/>
Undergraduate DNA nanotechnology research group from the University of Texas at Austin
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Results TODO
Fuel Test 1, 8-13-2012 Buffer: TMgK
Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, but this decreases noticeably in the absence of the initiator. From the presence of excess lines in both lanes that contain FD,h, it is clear that there are impurities in FD,h, which will require the test to be run again after the sample is purified.
Fuel Test 2, 8-14-2012 Buffer: TMgK
Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, regardless of whether the initiator is present. While this trend does decrease slightly when temperature of incubation is decreased and concentration of FA,h is increased, the change is not dramatic enough to make these hairpins viable as fuels in this buffer.
Fuel Test 2, 8-14-2012 Buffer: TNaK
Conclusion: There is significant hairpin leakage between FA,h and FB,h, and also between FA,h and FD,h, regardless of whether the initiator is present. There is no noticeable change in this trend when temperature of incubation is decreased or concentration of FA,h is increased. Therefore, these hairpins are not viable as fuels in this buffer.
